Background Central line-associated bloodstream infections (CLABSIs) create a huge burden of cost on healthcare organizations. Reducing the incidence of CLABSI will not only provide better healthcare and safety to patients but will also reduce the costs associated with additional days of stay. Central venous catheters are used in the healthcare system for a variety of indications, and bloodstream infections are one of the major complications. Central line bundles are a group of guidelines created to minimize and prevent infections acquired during administration. The purpose of our research was to assess the knowledge of central line bundles among healthcare workers in intensive care units.

Methods This cross-sectional study was done in King Abdulaziz Medical City in Riyadh and included 171 nurses and 41 physicians working in the three intensive care units (surgical, medical, cardiac). Participants’ awareness of central line bundles was assessed using a questionnaire that included three sections: demographics, knowledge, and practice.

Results The vast majority of the healthcare workers answered questions correctly regarding knowledge of CLABSI prevention (wearing maximal barrier precautions, washing hands, using chlorhexidine at insertion site, documenting the procedures, etc). The average knowledge score among participants was 82%. A major factor determining knowledge of central line bundles is training. 60% of the participants had received central line training. Participants who received CLABSI bundle achieved a high mean score of 84%, whereas this score was lower in those who had not received training (78%).

Conclusion Our results suggest that receiving CLABSI training is associated with better knowledge and lower complications rate. Educational interventions should be used to address the gaps regarding knowledge and practice regarding the prevention of CLABSI and to ensure that healthcare workers use evidence-based prevention interventions.