A regional Intensive Care Unit (ICU) with 10 beds and an average rate of Healthcare- Associated Infections (HAIs) of 40.9% was included in the PROADI-SUS/MS Health in Our Hands Project from 2021 to 2023, aiming to reduce rates by 30%. This work aims to demonstrate reductions in HAIs, increased hand hygiene (HH) adherence, process improvement, team engagement, and financial impact. A multidisciplinary core team was assigned to drive the project. Using the Institute for Healthcare Improvement (IHI) Model for Improvement methodology, driver diagrams were proposed, focusing on implementing prevention bundles to be tested through Plan-Do-Study-Act (PDSA) cycles, conducted at the bedside with participation from all shift teams. A Daily Management Board for Sustaining Improvement (DMBSI) was implemented as an assessment tool for adherence to work processes, identifying and planning improvement opportunities. This approach fostered a collaborative environment for developing a safety culture and team engagement. The initial baseline (LB) of 11.67 in Central Line-Associated Bloodstream Infection (CLABSI) was reduced by 100%. Ventilator-Associated Pneumonia (VAP) with LB 30.66 was reduced by 64%, Catheter-Associated Urinary Tract Infection (CAUTI) with LB 13.83 was reduced by 100%, and HH with LB 47.95 saw a 52% increase in adherence. The hospital participated in the cost model to measure the savings generated for SUS, estimating a saving of R$ 4,196,710.00 with 92 infections avoided. It is concluded that the applied methodology not only reduced HAIs, increased HH adherence, and team engagement but also has the potential to be disseminated to other sectors, impacting the quality of care, financial savings, and, most importantly, saving lives.